A study of biochemical composition, antibacterial activity and mineral elements content for Lancea tibetica / Монголын Анагаах Ухаан

ABSTRACT

Background@#Plants have played a significant role in maintaining human health and improving the quality of human life for thousands of years and have served humans as valuable components of seasonings, beverages, cosmetics, dyes and medicines. There are a number of medicinal plants used in the traditional medicine in the Mongolia to form “tan” prescriptions which are mostly consist of the mixture of individual medicinal plants and used by nomads during the long history for their health care as medicines. Lancea tibetica (Scrophulariaceae) is a traditional herbal medicinal plant in Mongolia, China and Tibetan. In Mongolian traditional medicine, L.tibetica different exclusive forms of preparations are effective for treatment of some disorders such as pulmonary, cough and heart disease. @*Aim@#This study aim was to define the biochemical some composition antibacterial activity and mineral elements content in air-dried samples Lancea tibetica of Mongolia. @*Material and Method@#The antibacterial activity and Minimal Inhibitory Concentration (MIC) of the extracts was collected of the seed, aboveground part and root for the Lancea tibetica. From three different organs of plant Lancea tibetica was collected of the Tsenkher district Arkhangai province (2017.07.28) and Tunel district Khuvsgul province (2018.08.27) were evaluated by means of the Disk diffusion method against five bacteria (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Micrococcus luteus, Enterococcus faecalis). We have taken extracts of plant 3 organs from organic solvent (7) ethyl acetate, hexane, n-butanol, methanol, chloroform, ethanol and distilled water for antibacterial activity. MIC of the extracts were defined and compared with each other. The macro-and microelements were determined by X-ray fluorescence spectrophotometer analysis (XRFA).@*@#@*Result@#We have taken a dried seed, aboveground part and root of Lancea tibetica in the investigation. In these three samples were determined biochemical composition of 11 components for quantitative analysis: moisture was 6.2±0.2%, ash 4.8%, protein content was 1.67±0.1%, raw oil 5.9±0.3%, reduction sugar 0.39±0.1%, vitamin Р 16.6±1.8 mg%, extractive substances 18.6±0.2%, tanning matter 0.05±0.02% and flavonoids 0.2±0.01% in seed. The same moisture was 6.7±0.4%, ash 9.7%, protein content 1.89±0.2%, raw oil 6.1±0.8%, reduction sugar 0.52±0.1%, vitamin С 3.52±2.2mg%, vitamin Р (rutin) 20.3±0.2mg%, carotene 12.8mg%, extractive substances 16.8±0.1%, tanning matter 0.08±0.01% and flavonoids 0.6±0.24% in aboveground part. Besides this, in root the moisture was 6.1±0.3%, ash was 14.9%, protein content was 2.1±0.3%, raw oil was 2.9±0.2%, reduction sugar 0.62±0.02%, vitamin Р 17.8±0.05mg%, extractive substances 20.3±0.4%, tanning matter was 0.06±0.03%, flavonoid was 0.35±0.002%. Besides this, we revealed the saponin by method quality analysis in aerial part and root of Lancea tibetica. But not triterpenoid saponin in seed. The same not revealed alkaloid all the samples. </br> Eighteen extracts (5mg/disc) of three organs (seed, aerial parts and root) showed antibacterial activity diverse. The MIC in ethanol and butanol extracts of seed, aboveground part and root was more than others. </br> From thoroughly air-dried aboveground part samples of Lancea tibetica from Tsenkher district of Arkhangai province and Tunel district of Khuvsgul province. We determined the 43 mineral elements. 11 elements were defined by percentage, 10 elements were oxide forms (SiO₂, TiO₂, Al₂O₃, Fe₂O₃, CaO, MgO, Na₂O, K₂O, MnO, P₂O₅) of them. Other elements were defined by mg/kg unit. The same heavy metallic, mobile elements (Cu, Cr, Zn, Mn, Pb, Ni, Co) and rare earth elements (Ce, La, Nd, Pr, Sm) were determined.@*Conclusion@#In the result of this research work to determine the content of biologically active substances in Lancea tibetica. Plants produce a great variety of organic compounds that are not directly involved in primary metabolic processes of ground and development. Study of the biochemistry of plant natural products has many practical applications. Various detectable contents of useful or macronutrient and micronutrient oxide was found in the ash of aerial parts. Some macronutrient and micronutrient elements were detected by oxide forms.