The study of toxicity (LD50) and immunomodulatory effect of antidiabet-3 preparation / Монголын Анагаах Ухаан

ABSTRACT

Introduction: There are over 1500 plants on our planet that have anti-diabetes properties. Research findings suggest that more than 400 plant species showing hypoglycemic activity on experimental diabetes in animals.Healthy diet, regular physical activity, maintaining a normal body weight and avoiding tobacco use can prevent or delay the onset diabetes. Recently, numbers of high level researches were conducted worldwide to study the nature and mechanism to treat diabetes, tens of methods were discovered, and dozens of medical herbs were studies, yet very few herbal hypoglycemic drugs without side effects and at low cost are found. Scientists are still in search for development of new and better oral drugs for diabetes without side effect at relatively low cost. Materials and Methods: The research was conducted at the Scientific Research Center of “Monos” Institute of Traditional Medicine and in biochemical Laboratory of “Khuljborjigon” Clinic. For the experiment, we used 23 perfectly healthy mice of same sex and size which meets standards of laboratory testing. The Prozorovski3 quick method for the determination of LD50 in the water (20%) and ethanol extractions (30%) of Antidiabetes-3 preparation (AD3). The tested animals were the white mice. Following Erne (1963), Kovalev I.E.,(1976), Petrov’s (1980) 4 methodology of studying effects on immune system, we have Antidiabetes-3 preparation (AD3) were given to the 33 mice 2 times a day in 3ml/200gr dose, during 7 days. On third day of the experiment, we injected into vein 2ml of 10 % sheep’s RBC to stimulate the immunity. On the fifth day, we defined weight of pancreas, number of pancreatic cells, pancreatic index, and haemagglutination titre to screen RBC antibodies.Results: The method developed by V.B. Prozorovski for the calculation of average lethal number was used on 40 white mice (18-22g). Water extraction (10%) was per fused in the tail vein of the experience mouse and the lethal dose (LD50) was 88.9g/kg. These facts prove that the toxic effect of the AD is low. The water (10%) extractions of “Antidiabetes-3” (AD3) preparation were given to the mice 2 times a day in 3ml/200g dose, during 8 days. We have studying compared group “Salimon and Immunal mixture” (S&I) to the mice 2ml/200g dose, during 8 days. On third day of the experiment, we injected into vein 2ml of 10 % sheep’s RBC to stimulate the immunity. On the fifth day, we defined weight of pancreas, number of pancreatic cells, pancreatic index, and hemagglutinin to screen RBC antibodies (Table 1). Figure 1 demonstrates increase in mice’s spleen weight on the 5th day after stimulation of immunity with sheep’s RBC antigen. Spleen weight increase in AD3 group was 1.6 times higher compare to control group (AD3 group 0.16±0.08; control group 0.10±0.02; p<0, 05), and AD3 group was 1.0 times level compare to control group (AD3 group 0.16±0.08; S&I group 0.17±0.09; p<0, 05). In figure 2, the spleen index in control group was 1.24 times higher than in normal group (control group 0, 0047±0.001; normal group 0.0038±0.0004; p<0, 3), AD3 group’s index was 1.3 times higher compare to control group (AD3 group 0.0061±0.002, control group 0, 0047±0.001; p< 0.05), and 1.0 times lower compare to S&I group (AD3 group 0.0061±0.002; S&I group 0.0062±0.003; p< 0.05). In figure 3, the number of spleen cells of control group’s was 142.71±55.51*106/ml. this is 1.2 times lower compare to normal group which is 172.67±135.5 *106/ml. AD3 group’s spleen cell number was 329.78±187.78*106/ml and 1.61 times bigger than in control group. In comparison to control group, haemagglutination titre of AD3 group was 1.13 times higher (AD3 group 54.86±19.95%; control group 50±8.83%, p<0,05) and this indicates that BV has immunity stimulating effect.Conclusions:1. Was defined the Antidiabet-3 preparation LD50, 88,9g/kg, its toxicity of classification (Sydorov K.K 1973) was little toxicity.2. Was defined to immunity stimulating effect the Antidiabet-3 preparation