Study result on anti-radical actvity and membrane endurance of otgontenger resorts / Монголын Анагаах Ухаан

ABSTRACT

Introduction: Although the physic-chemical nature and compounds of Otgontenger spring have been relatively studied over the last years, no any substantial scientific work was done in regard to its therapeutic action and significance.It is known that during gastritis, chronic hepatitis and rheumatism macro and micro elements and biologically active compounds dramatically decrease within cells of each related organ and the processes of cell’s bioenergy, bio-synthesis, and re-synthesis [1.2] are imbalanced that leads to loss of cell’s divisibility and its rejuvenescence. Consequently, disbalance in blood coagulation, micro-circulation, ischemia, hypothermia, reperfusion substantial decrease of endurance to anti-oxidant deficiency and anti-radical activity weakness are observed. So, our study has been done basing on our hypothesis that macro and micro elements as well as biologically active compounds in Otgontenger resorts as sulphate ion (SO42-), azotes (N2), hydrosulfuric acid (H2S), oxygen (О2), carbonate ion (СO32-), chloral ion (CL-), hydrogen (Н2), carbonic acid (СО2,) could replace the biologically regulative agents which are cardinally shortened and becoming the main factor of pathologies [3. 4].Goal The goal of this study is to investigate treatment activity “Otgontenger” resorts treatment experimental conditions. It is purposed to define the redoxy-potentiality and anti-radical activities of Otgontenger resorts.Materials and Methods: The study was conducted at the Central Scientific Laboratory of Monos Medical University between 2009-2011. Otgontenger mineral water was transported in a package accordingly to the Mongolian Standard “The Technical requirements to mineral water of treatment and drinking intent” (MNS 3651 2005). In order to determine the anti-radical activity of the mineral water each portion of the studying water is dissolved in ethanol. After that 0.1 and 0.2 mlg are taken from these solutions and are added to 1.5 mlg of alfa, alfa-demetilpicril-gydralice suspension. The solution is shaken well and once color deeply changed it was defined at 517 HM through spectrophotometer by B.Yu. Kulikov’s method (1995, 1996). In regard to Osmos’s endurance property, 16 white experimental rabbits are taken by mouth 1.0 mlg/200 grams each from the mineral water number 1 of the 1st group, number 18th of the 4th group and number 47th of the 7th group respectively two times a day for the consecutive five days. The blood is taken and centrifuged in a way that serum is segregated and 0.5 mlg of 10 percent erythrocytes is separated. Consequently, 5.0 mlg of distilled water is added and hymolize is carried out. Once gymolize is fully done, supernatant is separated after centrifuge and absorption is measured at 420hm by spectrophotometer. In regard to defining hydropereoxide endurance, the white rabbit is taken by mouth 1.0 mlg/200 grams each from the mineral water number 1 of the 1st group, number 18th of the 4th group and number 47th of the 7th group respectively two times a day for the consecutive five days. The blood is centrifuged and serum is segregated. The blood is taken and centrifuged in a way that serum is segregated and 0.5 mlg of 10 percent erythrocyte is separated. Consequently, 3.0 mlg of Н202 is added and hymolize is carried out. Once gymolize is fully done, supernatant is separated after centrifuge and absorption is measured at 420hm by spectrophotometer.Results: It has been defined that antiradical activity in the erythrocytes’ 4th compartment of the white rabbits taken mineral water number 18th of the 4th group and number 1st of the 1st group increased by 1.11-1.12 times compared to the group not taken, whereas, decreased by 1.1 times by the impacts of the mineral water under 47th number of the 7th group (Table 1). It is shown that the mineral water has effects to increase proton flows at mitochondria membranes and donators’ oxidation, because antiradical activity of the second compartment of the rabbit’s erythrocytes has gone down by 1.13-1.16 times compared to the group not taken because of the mineral water under number 18 of the 4th group and number 1 of the 1st group and number 47 of the 7th group. If we see table 2 and picture 2, it is proved that mineral water has effects to accelerate oxygen flow to erythrocytes or acceptors’ diffusion and as well energy supply because membrane’s peroxide endurance of erythrocytes’ fourth compartment of the white rabbit decreased by 1.11-1.12 times under the influence of Otgontenger resorts number 18 and 1 of the 4th and 1st groups respectively and as well osmos’s endurance property of membrane of the erythrocytes’ 4th compartment decreased by 1.2-1.22 times compared to those not taken it. If we see picture 3, it is proved that mineral water has effects to accelerate oxygen flow to erythrocytes or acceptors’ diffusion and energy supply because membrane’s peroxide endurance of erythrocytes fourth compartment of the white rabbit decreased by 1.11-1.12 times under the influence of Otgontenger resorts with numbers 18 and 1 of the 4th and 1st groups respectively and as well osmos’s endurance property of membrane of the erythrocytes’ 4th compartment decreased by 1.2-1.22 times compared to those not taken it.From picture 4 it can be seen that under the influence of some types of Otgontenger mineral water as 18th number of the 4th group and the first number of the 1st group MDA contained in the second compartment of the white rabbit decreases by 1.11-1.12 times compared to the control group not taken and as well osmoses’ endurance of the erythrocytes’ 4th compartment membrane declines by 1.2-1.22 times which means they have effects to accelerate oxygen flow to erythrocytes or acceptors’ diffusion and consequently energy supply. We can see from picture 5 that under influence of mineral water with number 47 of the 7th group, the oxidise-ferment activity of erythrocytes’ second compartment serum has decreased two times for the control group compared to the ones not taken the mineral water. Moreover, the oxidise-ferment activity of erythrocytes’ first compartment declined by 1.2 times as a result of the mineral water with number 47 of the 7th group. That means that the mineral water suppresses the ferment activity of lipoproteidlipaza but makes the flow of redoxy-line protons accelerated.Conclusion: It has been defined that Otgontenger mineral water has high antitoxic effects to reduce free radicals like DPHH.