Study of seasonal dynamics of phytoecdysteroids in silene repens / Монголын Анагаах Ухаан

ABSTRACT

Study of secondary plant products is important for understanding their functions and for obtaining biologically active compounds for practical use in different spheres of human activity. Among other plant substances, phytoecdysteroids attract great scientific interest, because of their potential contribution to crop protection strategies and their possible medical uses. Silene repens is widespread in Mongolia. Seed of Silene repens were collected in 2008 from Mongolian and Russian Altai and were cultivated in Siberian Botanical Garden at Tomsk State University, Russia.GoalStudy the dynamics of the 20-hydroxyecdysone in various plant organs of cultivated Silene repens.Material and MethodsThe seasonal dynamics of phytoecdysteroid content (20-hydroxyecdysone) observed in the vegetative and generative organs of cultivated Silene repens was investigated. The quantity of phytoecdysteroids was determined by spectrophotometer UV-1800 (Shimadzu, Japan).ResultsThe content of 20-hydroxyecdysone varied from 0.001 to 1.07 % depending on the organ and growth stage. The maximum content of 20-hydroxyecdysone was observed in actively developing parts of organs during beginning vegetation in roots (0.3 %); during budding, in the bud (1.00 %); during flowering, in the flowers (1.07 %); during fruiting, in roots (0.31 %). The general trends seen in the distribution of 20-hydroxyecdysone found for cultivated Silene repens from Mongolian and Russian Altai during the plant growth cycle. A high content of 20-hydroxyecdysone during flowering and a low amount during fruiting.ConclusionA high content of 20-hydroxyecdysone during flowering in the flowers and buds (1.07 -1,00 %) and low content of 20-hydroxyecdysone during fruiting in the stem (0.001-0.12 %). The seasonal dynamics of phytoecdysteroids in cultivated Silene repens has been studied for the first time.