“NEPHROPHYT” INFLUENCE ON THE COURSE OF EXPERIMENTAL / Монголын эм зүй, эм судлал

ABSTRACT

The data concerning the infl uence of the plant remedy on the functional state of kidneys, blood lipid spectrum, free radical processes activity and manifestation of immune disturbances in experimental glomerulonephritis are given in the present paper.Key words nephrophyt, Heiman’s nephritis, immune disturbances, dyslipidemia, free radicalprocesses. Kidney diseases of immune etiology are the main causes of the development of chronic kidney insuffi ciency resulting in invalidism and mortality. The immune disturbances take their toll in glomerulonephritis pathogenesis [1, 2]. The lipid metabolism impairments frequently noted in patients with chronic glomerulonephritis accompanied by nephritic syndrome are characterized by the increase of triglycerides (TG) in the blood, total cholesterol, low densitylipoproteins, non-esterifi cative fatty acids and atherogenic index. Experimental works by Bernard D.B. [3] have proved the injuring role of lipids in the development of kidney diseases. Free radical processes and lipid peroxidation are closely connected with lipid effects on the kidney tissue. These processes are heavily emphasized in nephrology due to their interrelation with infl ammation and cytomembrane destruction. At present the assortment of drugs used in chronic kidney insuffi ciency is limited; therefore, the search of new nephroprotective remedies is of great importance. The present work was aimed at the study of “nephrophyt” effect on the functional state of kidneys, lipid composition in the blood, free radical processes activity and manifestation of immune disturbances in the acute phase of experimental Heiman’s nephritis. The experiments were carried out on male and female Wistar rats weighing 180-200 g. The animals were maintained under standard laboratory conditions including regular care, diet, light and temperature regimen with free access to water. Experimental works were carried out observing the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientifi c Purposes (Strasbourg, 18 March 1986). Heiman’s nephritis was induced according to the J. Reynolds’ and C. Pusey’s method modifi ed by I.V. Mukhin’s [4]. The animals of the experimental group received “nephrophyt” at the dose of 150 mg/kg a day for 3 weeks since the last introduction of the cortical layer suspension with a full Freind’s adjuvant. “Nephrophyt” is a complex of dry extracts derived from the herb of a common knotgrass (Polygonum aviculare L.), leaves of a common bearberry (Arctostaphylos uva ursi L.), fl ushes of Orthosiphon stamineus (Orthosiphon stamineus Benth.), herb of Canada tickclover (Desmodium canadense (L.) D.S.).