ACETHYLCHOLINESTERASE INHIBITORY ACTIVITY OF SOME MONGOLIAN PLANTS / Монголын эм зүй, эм судлал

ABSTRACT

Introduction: Alzheimer’s disease(AD) is the most common neurodegenerative disease and characterized by memory impairment, language deterioration, cognitive dysfunction, behavioral disturbances and deficits in activities in daily living [1,2]. Acetylcholinesterase(AchE) inhibitors have been used effective treatment for AD disease targets[3]. Therefore, it is needed to isolate bioactive compounds from plants.Materials and methods: Trisma buffer pH 8.0, DTNB(5.5-dithiobis-2-nitribenzoic acid), acethylcholinesterase enzyme, acethylcholin iodide- Ach and methanol reagents were used. Plants were collected from Mongolian khangai, steppe, gobi regions. The dried plant material were extracted with methanol then evaporiated at 400C in vacu. AChE inhibitory activity was determined using the Ellman’s colorimetric method.CalculationA10 min-A00min= Δ A Inhibition % =100x (A10 control- Δ A sample) A10control A=absorptionResults and discussion: From the 65 samples studied, which prepared from different parts of 44 plants species, Salsola passerine, Rumex pseudonatronatus, Solidago dahurica, Chelidonium majus, Anthriscus sylvestris, Linaria buriatica and Achnatherum splendens showed more effect, their inhibition percentages were 32%, 30%, 30,7%, 35,7%, 61,5%, 29,8%, 28.8% and26.6%, respectively. Out of the plants, eight extracts were not identified AChE inhibitory activity and 30 extracts showed 10-20% weak inhibition ratios. This work represents some Mongolian plants have AChE inhibitory activity.Referens1. Marston A., Kissling J., Hostettmann K., A rapid TLC bioautographic method for the detection of acetylcholinesterase and butyrylcholinesterase inhibitors in plants.Phytochem.Anal. 13.51- 54(2002)2. Eduardo luis Konrath., Bruna Medeiros Neves et all., Investigations of the in vitro and in vivo acetylcholinesterase and antioxidant activities of tradionally used Lycopodium species from South America on alkaloid extracts. Journal of Ethnoparmacology 139(2012)58-673. Shulz V., Ginkgo extract or cholinesterase inhibitors in dementia., Phytomedicine 10(2003)74-79