Bone Marrow Mesenchymal Stem Cells Differentiated into Hepatocyte-like Cells Induced by Rebirth Liver Tissue Lixivium of Mouse in Vitro / 中国康复理论与实践
Chinese Journal of Rehabilitation Theory and Practice
;
(12): 1056-1057, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-977564
ABSTRACT
@#Objective To induce bone marrow mesenchymal stem cells (BMSCs) differentiated into hepatocyte-like cells with rebirth liver tissue lixivium of mouse in vitro.Methods Mouse BMSCs were isolated and directionally induced with rebirth liver tissue lixivium of mouse 36 h after partial hepatectomy (PH). The morphology of cells was observed under an invert microscope, and the characteristics of differentiated cells was identified by immunofluorescence test.Results 7 days after induced by liver lixivium, the spindle shaped BMSCs became round and resembled hepatocyte-like cells. 1~2 weeks later, the differentiated cells expressed albumin, CK8 and CK18, which were known as characteristic markers of the hepatocyte.Conclusion BMSCs of mouse can be differentiated into hepatocyte-like cells under induction of liver tissue lixivium of mouse.
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Rehabilitation Theory and Practice
Year:
2006
Type:
Article
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