Effects of Different Astrocytes on Differentiation of Neural Stem Cells / 中国康复理论与实践
Chinese Journal of Rehabilitation Theory and Practice
;
(12): 1026-1028, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-977736
ABSTRACT
@#Objective To observe the differentiation of neural stem cells(NSC)into neurons under different astrocytes feeding layers.MethodsThe NSC purified from primary cultured clones and labeled by DAPI in serum-free media were plated in different feeding layerscyto-plasma astrocytes and fibrous astrocytes respectively with Neural Basal(NB)media.After 10 d,immunohistochemistry with antibody NF-200 was taken to calculate the percents of neurons by 20 fields of vision chosen randomly.The differentiated neurons were stained with AchE and labeled with Fura-3AM.ResultsThe ratios of differentiated neurons in cyto-plasma and fibrous astrocytes were 72% and 43% respectively.In most differentiated neurons the AchE staining was positive and had the activity of Ca2+ stimulated by medicine.ConclusionThe cyto-plasma astrocytes can induced NSC differentiated into neurons,especially into active motor neurons,which can be chosen for a new seeding cells in the nervous tissue engineering.
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Rehabilitation Theory and Practice
Year:
2007
Type:
Article
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