Effect of Earle's solution and MEM on activities of neurons in culture / 中国康复理论与实践
Chinese Journal of Rehabilitation Theory and Practice
; (12): 125-126, 2005.
Article
in Zh
| WPRIM
| ID: wpr-977964
Responsible library:
WPRO
ABSTRACT
@# ObjectiveTo observe the activities of cultured neurons incubated with Earle's solution, new minimal essential medium (MEM) or former MEM.MethodsActivity changes of cultured neurons were measured by determining the leakage of lactate dehydrogenase (LDH) and metabolic rate of 3-(4,5-dimethylthiazol)2,5 diphenyltetrazolium bromide (MTT).ResultsActivities of neurons incubated with different culture medium for 12 h or 24 h were significantly different (P<0.01).ConclusionDifferent culture medium can influence neuronal activities.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Rehabilitation Theory and Practice
Year:
2005
Type:
Article