Change of free radical in serum and the expression of Bax, Bcl-2 and p53 in the liver after ischemia-reperfusion of small intestine / 中国康复理论与实践

ABSTRACT

@#ObjectiveTo study the injuries of liver after ischemia-reperfusion of small intestine of the rat.MethodsModels of ischemia-reperfusion of small intestine was made with rats. At 0 min, 30 min, 1h, 2h, 1d, 3d,7d after reperfusion, the concentration of nitric oxide(NO), superoxide dismutase (SOD) in the serum was examed and the expression of Bax, Bcl-2 and p53 in the liver was observed by the immunohistochemical SP method.ResultsThe concentration of NO increased apparently 0 min after reperfusion, but decreased 2 h after, then increased gradually to a peak at 7th day. But for SOD, the concentration decreased 0 min after reperfusion, increased 2 h after,and decreased to the lowest level at 7th day. The immunohistochemical SP positive cells were observed in sinus endothelial cells and hepatocytes. The ratio of positive cells of Bax,p53 and Bcl-2 began to increase 0 minute after reperfusion and increased continuously 30 min after, while that of Bcl-2 was higher than that of Bax(P<0.01). It decreased apparently 2h after, and then increased till 7d after reperfusion,while the ratio of Bax positive cells was higher than that of Bcl-2(P<0.01).ConclusionThe change of concentration of NO, SOD and the expression of positive cells of Bax, Bcl-2 and p53 might play a important role in apoptosis and injuries of the liver after ischemia-reperfusion of small intestine of rat.