Pro-culture with serum promoting neural stem cell proliferation / 中国康复理论与实践
Chinese Journal of Rehabilitation Theory and Practice
;
(12): 339-340, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-978103
ABSTRACT
@# ObjectiveTo introduce a method of promoting neural stem cells proliferation.MethodsNeural stem cells were cultured with medium DMEM containing 10% fatal bovine serum for 48 h, then changed medium DMEM/F12 containing epidermal growth factor, basic fibroblast growth factor and B27. Immunohistochemistry was used to identify nestin expression.ResultsNeural stem cells got together fast and formed neurospheres within 48 h and expressed nestin. ConclusionPro-culture with serum can promotes neural stem cell proliferation.
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Rehabilitation Theory and Practice
Year:
2005
Type:
Article
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