Signal transduction of cytokines in inducing matrix metalloproteinas-9 in myometrial smooth muscle cells from term pregnant women / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 938-945, 2001.
Article
in Korean
| WPRIM
| ID: wpr-98023
ABSTRACT
OBJECTIVE:
To explore the intracellular signal transduction pathways of IL-1beta and TNF-alpha in inducing matrix metalloproteinase-9 (MMP-9) in human myometrial smooth muscle cells.METHODS:
We studied the expression of MMP-9 induced by cytokines (TNF-alpha and IL-1beta) with zymography. The influence of TNF-alpha and IL-1beta on the phosphorylation of Jun N-terminal kinase (JNK) and IkB were studied with immunoblotting for p-JNK and p-IkB. The intranuclear shifting of NF-kB and AP-1 after treatment with TNF-alpha and IL-1beta were evaluated by EMSA.RESULTS:
TNF-alpha- and IL-1beta-induced MMP-9 expression was not suppressed by NF-kB inhibitor (CAPE), AP-1 inhibitor (curcumin) and PKC inhibitor (calphostin C) but was inhibited by tyrosine kinase inhibitor (genistein). After treatment of myometrial smooth muscle cells with TNF-alpha and IL-1beta, phosphorylation of JNK and phosphorylation of IkB with degradation of IkB were evidently observed. The intranuclear translocations of NF-kB and AP-1 were strongly enhanced after treatment with TNF-alpha and IL-1 beta as demonstrated in EMSA.CONCLUSION:
In myometrial smooth muscle cells, MMP-9 is induced by TNF-alpha and IL-1beta through PKC activation and transcriptional activations of NF-kB and AP-1. Independent of PKC activation, the signaling of TNF-alpha and IL-1beta in the induction of MMP-9 seems to be transmitted by way of either NF-kB or AP-1 activation in myometrial smooth muscle cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphorylation
/
Phosphotransferases
/
Uterus
/
Protein-Tyrosine Kinases
/
Immunoblotting
/
Signal Transduction
/
Cytokines
/
NF-kappa B
/
Tumor Necrosis Factor-alpha
/
Transcription Factor AP-1
Limits:
Female
/
Humans
/
Pregnancy
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2001
Type:
Article
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