Isolation and identification of a polyester-polyurethane degrading bacterium Bacillus altitudinis YX8-1 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1976-1986, 2023.
Article
in Chinese
| WPRIM
| ID: wpr-981183
ABSTRACT
Although polyurethane (PUR) plastics play important roles in daily life, its wastes bring serious environmental pollutions. Biological (enzymatic) degradation is considered as an environmentally friendly and low-cost method for PUR waste recycling, in which the efficient PUR-degrading strains or enzymes are crucial. In this work, a polyester PUR-degrading strain YX8-1 was isolated from the surface of PUR waste collected from a landfill. Based on colony morphology and micromorphology observation, phylogenetic analysis of 16S rDNA and gyrA gene, as well as genome sequence comparison, strain YX8-1 was identified as Bacillus altitudinis. The results of high performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) showed that strain YX8-1 was able to depolymerize self-synthesized polyester PUR oligomer (PBA-PU) to produce a monomeric compound 4, 4'-methylene diphenylamine. Furthermore, strain YX8-1 was able to degrade 32% of the commercialized polyester PUR sponges within 30 days. This study thus provides a strain capable of biodegradation of PUR waste, which may facilitate the mining of related degrading enzymes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Polyesters
/
Polyurethanes
/
Bacteria
/
Biodegradation, Environmental
/
Chromatography, Liquid
/
Tandem Mass Spectrometry
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2023
Type:
Article
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