Soybean GmGolS2-2 improves drought resistance of transgenic tobacco / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 2762-2771, 2023.
Article
in Chinese
| WPRIM
| ID: wpr-981231
ABSTRACT
Galactinol synthase (GolS) genes play important roles in plant response to abiotic stress. In this research, the plant expression vector of soybean GmGolS2-2 gene was constructed and transformed into tobacco to study the drought tolerance of transgenic tobacco. A GmGolS2-2 gene with 975 bp coding sequence was cloned from soybean leaves by reverse transcription-polymerase chain reaction (RT-PCR). GmGolS2-2 was linked to the plant expression vector pRI101 by restriction enzyme sites Nde Ⅰ and EcoR Ⅰ, and transformed into tobacco by leaf disc method. Genomic DNA PCR and real-time PCR showed that three GmGolS2-2 transgenic tobacco plants were obtained. The growth status of GmGolS2-2 transgenic tobacco under drought stress was better than that of wild-type tobacco. After drought stress treatment, the electrolyte leakage and malondialdehyde content of transgenic tobacco were lower than those of wild-type tobacco, but the proline content and soluble sugar content were higher than those of wild-type tobacco. The results of real-time PCR showed that the heterologous expression of GmGolS2-2 increased the expression of stress-related genes NtERD10C and NtAQP1 in transgenic tobacco. The above results indicated that GmGolS2-2 improved drought resistance of transgenic tobacco.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plant Proteins
/
Soybeans
/
Stress, Physiological
/
Tobacco
/
Plants, Genetically Modified
/
Gene Expression Regulation, Plant
/
Droughts
/
Drought Resistance
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2023
Type:
Article
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