New improved approaches for DYS385 detection / 法医学杂志
Journal of Forensic Medicine
;
(6): 27-29, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-982965
ABSTRACT
OBJECTIVE@#To establish more sensitive methods for detection of DYS385 in routine forensic casework.@*METHODS@#The primers recommended by Genome Database (GDB) and Schneider were used to amplify DYS385 respectively. Then, a semi-nested PCR of DYS385 was designed by using the two different primers as outer and inner primer. A series of experiments were carried out to achieve good result by adjusting the ratio of outer/inner primer and optimizing the PCR condition.@*RESULTS@#It showed that an overall 112 bp shorter DYS385 fragments and better electrophoretic separation were obtained by using primer2B. By using the semi-nested PCR approach, the shorter specific DYS385 fragments could be amplified and detectable DNA amounted to 50 pg.@*CONCLUSION@#This method is 20 fold more sensitive than the ordinary method.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymerase Chain Reaction
/
Sequence Analysis, DNA
/
Tandem Repeat Sequences
/
Chromosomes, Human, Y
/
Forensic Medicine
/
Gene Frequency
Limits:
Humans
/
Male
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2003
Type:
Article
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