Genotype reliability of short tandem repeats typing from minute DNA / 法医学杂志
Journal of Forensic Medicine
;
(6): 151-153, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-982990
ABSTRACT
OBJECTIVE@#To understand the forensic practice of DNA profiling from minimal amount of DNA.@*METHODS@#Serial dilutions of DNA were amplified with the PowerPlex 16 System Kit, then the genotyping of short tandem repeat(STR) was performed by ABI 377 DNA automated Sequencer.@*RESULTS@#When the mount of DNA template was less than 250 pg, allelic drop-out apparently occurred at several loci. Other disturbed peaks, such as artefact bands and imbalanced heterozygote, also presented.@*CONCLUSION@#The anomalous results may result in incorrect genotyping. Careful and comprehensive considerations are needed to interpret the STR profile of minute DNA.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA
/
Sequence Analysis, DNA
/
Minisatellite Repeats
/
Tandem Repeat Sequences
/
Alleles
/
Forensic Medicine
/
Genotype
Limits:
Humans
/
Male
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS