A sort of meliorative method for testing DNA from sperm smear / 法医学杂志

ABSTRACT

OBJECTIVE@#To establish an effective method for testing DNA from the sperm smear.@*METHODS@#We did exploratory research for ninety-one cases of the sperm smear which were accumulated by our laboratory during working hours. Chelex extraction method was used to extract DNA. The digested solution of sperm was purified and concentrated by using Silicon bead. STR loci were typed after PCR amplification by Profile Plus kit.@*RESULTS@#Although there were a few sperm cells on the slide, it is easy to obtain good DNA typing result from it because the amalgamative method we used was high effective for DNA extraction.@*CONCLUSION@#The DNA analysis of sperm smear could offer satisfactory typing results which would be useful in case.