Detecting haplotypes of four Y-STR by multiplex amplification / 法医学杂志
Journal of Forensic Medicine
;
(6): 9-14, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-983061
ABSTRACT
OBJECTIVE@#To establish a multiplexing Y-STR system and study haplotype frequencies of 4 Y-specific loci in China Han population.@*METHODS@#DYS439, DYS390, GATA-A7.2 and DYS393 loci were amplified simultaneously and were analyzed by polyacrylamide gel electrophoresis and silver staining.@*RESULTS@#When 558 unrelated male individuals from the Han population in China were tested by the multiplex system, DYS439, DYS390, GATA-A7.2 and DYS393 show 7,7,7,6 alleles, respectively. 180 different haplotypes were detected. The power of discrimination of this system was 0.9853.@*CONCLUSION@#The multiplex amplified system of these 4 Y-specific loci and their database are useful for human origin exploration and forensic practice.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymorphism, Genetic
/
Haplotypes
/
DNA
/
Ethnicity
/
China
/
Polymerase Chain Reaction
/
Silver Staining
/
Tandem Repeat Sequences
/
Chromosomes, Human, Y
/
Electrophoresis, Polyacrylamide Gel
Limits:
Female
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2005
Type:
Article
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