Forensic applications of the sequencing of mitochondrial DNA cytochrome oxidase subunit I gene for sarcosaphagous flies (Diptera) in Huhhot and Dunhuang district / 法医学杂志

ABSTRACT

OBJECTIVE@#To solve the problems of identification of Sarcosaphagous flies and their larvae, pupas and eggs.@*METHODS@#Sarcosaphagous Flies (Diptera) Samples were collected on the corpses of rabbits in the Huhhot district and a pig in the Dunhuang district. A 278bp region in the cytochrome oxidase subunit I (CO I) gene in mtDNA was analysed by DNA sequencing, A neighbour-joining tree using the Tamura and Nei model of nucleotide substitution was also constructed using the MEGA2.1 package.@*RESULTS@#A 278 base pairs region of the gene for CO I encoding region of mtDNA of above all samples was showed less than 1% sequence divergence within species and about 3% divergence between species.@*CONCLUSION@#It is an effective, easy and accurate method to be used for identification of these Sarcosaphagous Flies (Diptera) to species group by sequencing the 278 base pairs region of the CO I encoding gene of mtDNA.