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A study on the relationship between the degradation of protein and the postmortem interval / 法医学杂志
Journal of Forensic Medicine ; (6): 110-112, 2005.
Article in Chinese | WPRIM | ID: wpr-983084
ABSTRACT
OBJECTIVE@#To observe the degradation of actin and tubulin in the liver tissue of rats after death and to find an objective indicator of the postmortem interval (PMI).@*METHODS@#Female rats were killed under anesthesia by ether and incubated at 21 degrees C in a temperature controlled system to simulate postmortem changes for 18 days postmortem. Protein in the hepatic tissue was extracted, actin and tubulin were then examined by western blot. Thereafter, the semi-quantitative analysis of the image of western blot was performed.@*RESULTS@#Actin in the liver tissue of rats could be detected at 8 days postmortem, but could not be examined after 10 days postmortem. beta-tubulin rather than alpha-tubulin could be examined after 2 days postmortem, and beta-tubulin could not be examined at 4 days postmortem.@*CONCLUSION@#There is some difference in the degradation between actin and tubulin, their different preservation period postmortem may be used as a parameter for PMI estimation.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Postmortem Changes / Time Factors / Tubulin / Blotting, Western / Actins / Rats, Sprague-Dawley / Forensic Medicine / Liver Limits: Animals Language: Chinese Journal: Journal of Forensic Medicine Year: 2005 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Postmortem Changes / Time Factors / Tubulin / Blotting, Western / Actins / Rats, Sprague-Dawley / Forensic Medicine / Liver Limits: Animals Language: Chinese Journal: Journal of Forensic Medicine Year: 2005 Type: Article