Constructing of STR slippage model by optimizing some factors / 法医学杂志
Journal of Forensic Medicine
;
(6): 39-42, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-983126
ABSTRACT
OBJECTIVE@#To construct STR slippage model and study factors involved in this procedure.@*METHODS@#DNA samples were amplified with the technology of Degenerate oligonucleotide- primed PCR, then their products were taken as later DNA template and their STR genotype were analyzed by optimizing several factors.@*RESULTS@#STR slippage model was constructed.@*CONCLUSION@#Several factors were involved in the produce of STR slippage, such as amount of modulate DNA, concentration of MgCl2, property of DNA polymerase, motif sequence of STR loci, sample, etc.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Genetic Variation
/
DNA
/
Genetic Markers
/
Polymerase Chain Reaction
/
DNA Primers
/
Tandem Repeat Sequences
/
Genotype
/
Magnesium
/
Models, Genetic
Limits:
Humans
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2006
Type:
Article
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