Polymorphisms analysis of mitochondrial DNA in coding area / 法医学杂志
Journal of Forensic Medicine
;
(6): 45-47, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-983128
ABSTRACT
OBJECTIVE@#To analyze mitochondrial DNA (mtDNA) polymorphisms in coding area and provide a theoretical basis for applying in forensic science.@*METHODS@#The primers of 8162F/8483R and 13070F/13299R were designed according to the Anderson's sequence. Using PCR-sequencing method to detect polymorphisms of mtDNA nt8162-8483 and nt13070-13299.@*RESULTS@#The lengths of the amplicons were 322 bp and 230 bp respectively. There were 24 mitochondrial haplotypes defined by 21 variable positions in both regions. The gene diversity was estimated at 0.751 1, and the probability of two randomly selected individuals having identical mtDNA types was 0.256 4.@*CONCLUSION@#The polymorphic sites within mtDNA coding area can be useful in combination with mtDNA control region in order to increase the discrimination power.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Genetic Variation
/
DNA, Mitochondrial
/
Base Sequence
/
Polymerase Chain Reaction
/
DNA Fingerprinting
/
Sequence Analysis, DNA
/
DNA Primers
/
Polymorphism, Single Nucleotide
/
Genotype
/
Haploidy
Limits:
Humans
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2006
Type:
Article
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