Determination of opiates in biological human samples by liquid chromatography-tandem mass spectrometry / 法医学杂志
Journal of Forensic Medicine
;
(6): 52-57, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-983130
ABSTRACT
OBJECTIVE@#Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of opiates in biological samples according to the emerging problem in drugs abuse.@*METHODS@#Opiates such as heroin, 6-acetylmorphine, morphine, codeine, acetylcodeine, hydrocodone and hydromorphone were isolated from human blood, urine, oral fluid and hair using a simple extraction and consequently analyzed using LC-MS/MS. The method was evaluated by real cases.@*RESULTS@#The mobile phase give the optimum separation for opiates. The detection limit of morphine in urine with dilution and liquid-liquid extraction and in hair is 10ng/mL, 0.01 ng/mL and 0.01 ng/mg, respectively.@*CONCLUSION@#The method is simple and rapid, offering superior sensitivity and selectivity for opiates. The target compounds comprising hydrocodone and hydromorphone enlarge the applied area.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Saliva
/
Substance Abuse Detection
/
Reproducibility of Results
/
Chromatography, Liquid
/
Codeine
/
Hydromorphone
/
Tandem Mass Spectrometry
/
Forensic Medicine
/
Hair
/
Hydrocodone
Limits:
Humans
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2006
Type:
Article
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