Identification of six species of sarcosaphagous flies (Diptera) by sequence analysis of cytochrome oxidase subunit I gene (COI) in Weifang

Xin-Jie WANG; Xue-Hai WANG; Li-Jiang DIAO; Gui-Ping LU

Identification of six species of sarcosaphagous flies (Diptera) by sequence analysis of cytochrome oxidase subunit I gene (COI) in Weifang / 法医学杂志

Xin-Jie WANG; Xue-Hai WANG; Li-Jiang DIAO; Gui-Ping LU.

Journal of Forensic Medicine ; (6): 93-94, 2006.

Article in Chinese | WPRIM | ID: wpr-983147

ABSTRACT

ABSTRACT

OBJECTIVE@#To identify sarcosaphagous flies and their larvae, pupa.@*METHODS@#Sarcosaphagous flies and their larvae, pupas were collected from human corpses and their surroundings in the Weifang city. A 304 bp region in COI gene was analyzed by mtDNA sequencing.@*RESULTS@#The studied region showed no sequence divergence within same species and significant difference were found between different species in all samples.@*CONCLUSION@#It is a practical approach to identify these Sarcosaphagous flies and their larvae, pupas by sequence analysis of the 304bp region of the COI in mtDNA.

Subject(s)

Animals; Humans; Base Sequence; China; DNA Primers; DNA, Mitochondrial/genetics; Diptera/genetics; Electron Transport Complex IV/genetics; Forensic Medicine; Genes, Insect; Larva/genetics; Phylogeny; Polymerase Chain Reaction/methods; Pupa/genetics; Sequence Analysis, DNA/methods; Species Specificity

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Pupa / Species Specificity / DNA, Mitochondrial / Base Sequence / China / Polymerase Chain Reaction / Sequence Analysis, DNA / Genes, Insect / DNA Primers Limits: Animals / Humans Country/Region as subject: Asia Language: Chinese Journal: Journal of Forensic Medicine Year: 2006 Type: Article

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