Developing of a new multicolor-fluorescent labeled STR amplification kit / 法医学杂志

ABSTRACT

OBJECTIVE@#To develop a PCR-based STR system for genotyping of 18 loci (Amelogenin, D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D16S539, TH01, TPOX, CSF1PO, D7S820, D2S1338, D19S433, D12S391 and D19S253).@*METHODS@#By using primers labeled with four color fluorescent (FAM, HEX, TAMRA and ROX), two multiplex amplification reaction systems were developed to genotype Amelogenin and 17 STR loci.@*RESULTS@#Amelogenin and these 17 STR loci were genotyped successfully in different kinds of biological samples by the kit.@*CONCLUSION@#The STR amplification kit developed in our study gives a new approach to genotype these 18 loci in a efficient, steady and reliable way.