The application of PCR-SSCP in forensic mtDNA typing / 法医学杂志
Journal of Forensic Medicine
;
(6): 117-119, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-983154
ABSTRACT
OBJECTIVE@#To study the application of PCR-SSCP in forensic mtDNA typing.@*METHODS@#Primers flanking the mtDNA HV-I and HV-II regions were designed. By PCR-SSCP techniques, 70 family trios and 140 unrelated Wuhan Han individuals were investigated and analyzed.@*RESULTS@#In 70 family trios, the SSCP profiles in region HV-I and HV-II of children were not same to that of their fathers in 98.57% and 97.13% respectively but were identical with their mothers. In 140 unrelated Wuhan Han individuals, 21 haplotypes were found in HVI, GD = 0.9556; 16 haplotypes were found in HVII, GD = 0.9356.@*CONCLUSION@#PCR-SSCP technique may be useful in forensic mtDNA typing, especially for screening the suspects.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pedigree
/
Haplotypes
/
DNA, Mitochondrial
/
Polymerase Chain Reaction
/
DNA Fingerprinting
/
Sequence Analysis, DNA
/
DNA Primers
/
Polymorphism, Single-Stranded Conformational
/
Forensic Genetics
Limits:
Humans
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS