A PCR-RFLP analysis to HLA-B gene among Chinese northern Han populations / 法医学杂志
Journal of Forensic Medicine
;
(6): 193-195, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-983178
ABSTRACT
OBJECTIVE@#To set up the method for analyzing HLA-B gene polymorphism with PCR-RFLP, and to gain population data among northern Chinese Hans of HLA-B's restricted fragments after NlaIII digestion, and to achieve application in forensic medicine practice.@*METHODS@#Sample DNA was extracted by the phenol/chloroform extraction method, 943 bp-long fragments containing HLA-B exon 2 and 3 were got by PCR. The endonuclease NlaIII was applied to cut the PCR products into polymorphic fragments shorter than 943bp, then PAGE and silver staining were used to detect the digestion results, finally the digestion sites were assured by DNA sequencing.@*RESULTS@#Along 943bp-long PCR products, 14 length-different fragments, 20 kinds of fragment combinations were got and 6 cutting site were observed after NlaIII digestion.@*CONCLUSION@#HLA-B gene was highly polymorphic among Chinese northern Hans. Even with only one endonuclease, 14 restricted fragments were got and the PIC was great. Such a HLA-B PCR-RFLP analysis will have values in forensic medicine applications.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymorphism, Restriction Fragment Length
/
DNA
/
Base Sequence
/
HLA-B Antigens
/
China
/
Polymerase Chain Reaction
/
Exons
/
Asian People
/
Forensic Medicine
/
Gene Frequency
Limits:
Humans
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2006
Type:
Article
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