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DNA genotyping of oral epithelial cells by laser capture microdissection / 法医学杂志
Journal of Forensic Medicine ; (6): 196-203, 2006.
Article in Chinese | WPRIM | ID: wpr-983179
ABSTRACT
OBJECTIVE@#The STR genotypping of trace oral epithelial cells which are microdissected by laser capture microdissection system (LCM) is explored.@*METHODS@#The oral epithelial cells are microdissected using a low-power infrared laser by VERITAS Microdissection Instrument. STR loci of Profiler Plus are detected by multiplex PCR procesures.@*RESULTS@#DNA genotyping of 7-8 oral epithelial cells are succeeded, and DNA genotyping of 3-4 oral epithelial cells are failed.@*CONCLUSION@#It is viable in genotyping of trace oral epithelial cells by Laser Capture Microdissection as a new technology of seperating single cell.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: DNA / Cell Separation / Tandem Repeat Sequences / Microdissection / Epithelial Cells / Genotype / Lasers / Mouth Limits: Humans Language: Chinese Journal: Journal of Forensic Medicine Year: 2006 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: DNA / Cell Separation / Tandem Repeat Sequences / Microdissection / Epithelial Cells / Genotype / Lasers / Mouth Limits: Humans Language: Chinese Journal: Journal of Forensic Medicine Year: 2006 Type: Article