Analysis of 37 drugs in whole blood by HPLC after solid phase extraction / 法医学杂志
Journal of Forensic Medicine
;
(6): 349-352, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-983221
ABSTRACT
OBJECTIVE@#To develop a specific, sensitive, reproducible SPE-HPLC method for the determination of 37 drugs in whole blood.@*METHODS@#With the doxapram as internal standard, Oasis column was used to extract drugs from whole blood. Two kinds of mobile phases were used in this study. Separations were achieved by a LiChrospher 100 RP-C18 (250 mm x 4.0 mm x 5 microm) column kept at 50 degrees C, the DAD detector was set at 230 nm and 250 nm.@*RESULTS@#The limit of detection were 1-30 ng/mL. The method showed excellent linearity and the linear correlation coefficient was > or =0.997 98. The relative standard deviation for between-day and within-day assay were <10%.@*CONCLUSION@#The method is effective, simple, reliable and has been used in real cases.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Papaverine
/
Procaine
/
Solvents
/
Prazosin
/
Pharmaceutical Preparations
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Sensitivity and Specificity
/
Doxapram
/
Doxepin
Limits:
Humans
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2006
Type:
Article
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