Expression of cathepsin-B and -D in rat's brain after traumatic brain injury / 法医学杂志
Journal of Forensic Medicine
;
(6): 404-410, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-983233
ABSTRACT
OBJECTIVE@#To study the expression of cathepsin-B and -D in different time point after traumatic brain injury.@*METHODS@#Traumatic brain injury (TBI) model was established on rats, cathepsin-B and cathepsin-D immunofluorescence staining and confocal microscope analysis were performed. Positive cells were counted by confocal microscope and image analysis techniques were used to determine the morphological changes in each group.@*RESULTS@#Immunofluorescence staining results showed that cathepsin-B was activated 1 hour after TBI while cathepsin-D was not activated until 12hour after TBI. Both of them got to their peak during 4 to 8days, and kept a high level of activating 32days after TBI. Cathepsin-B and -D positive cells did not merge with caspase-3 positive cells until 6 h after TBI.@*CONCLUSION@#Cathepsin-B and -D could be the diagnostic markers of TBI and can estimating time course of lateral TBI. They blocked caspase-3 activation at the beginning period after TBI and started to promote cell death with caspase-3 6 h after TBI.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Time Factors
/
Brain
/
Brain Injuries
/
Immunohistochemistry
/
Cathepsin B
/
Cathepsin D
/
Rats, Sprague-Dawley
/
Forensic Pathology
/
Disease Models, Animal
/
Caspase 3
Limits:
Animals
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS