DNA genetyping of the trace bloodstains on the adsorbent object / 法医学杂志
Journal of Forensic Medicine
;
(6): 302-303, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-983305
ABSTRACT
OBJECTIVE@#To study the extraction and analysis of DNA from trace bloodstains on the adsorbent object.@*METHODS@#DNA was extracted by Chelex-100, QIAamp Mini Kit, and modified QIAamp Mini Kit, respectively. The extracted DNA was amplified and short tandem repeat (STR) loci were genotyped by multiplex PCR procedures, and then analyzed by ABI 3100 Genetic Analyzer.@*RESULTS@#The STR loci could be better genotyped with modified QIAamp Mini Kit, compared to poorer genotyping results obtained with Chelex-100 extraction method and QIAamp Mini Kit.@*CONCLUSION@#Our data indicate that DNA extracted from trace bloodstains on adsorbent object with modified QIAamp Mini Kit serves a better DNA template. for amplification and genotyping.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Resins, Synthetic
/
Specimen Handling
/
Blood Stains
/
DNA
/
Polymerase Chain Reaction
/
DNA Fingerprinting
/
Clothing
/
Tandem Repeat Sequences
/
Forensic Medicine
Limits:
Humans
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2007
Type:
Article
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