Effects of serum from crush injury rats on vascular endothelial cell apoptosis and their potential mechanism / 法医学杂志
Journal of Forensic Medicine
;
(6): 409-413, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-983327
ABSTRACT
OBJECTIVE@#To investigate the effects of serum from crush injury rats on vascular endothelial cell apoptosis and their potential mechanism.@*METHODS@#Bovine aorta endothelial cells were cultured in vitro and the effects of serum from crush injury rats on cell apoptosis and intracellular calcium concentration ([Ca2+]i) were observed. Meanwhile, the levels of rat blood plasma endothelin-1 (ET-1) and atrial natriuretic peptide(ANP) were measured.@*RESULTS@#Compared with normal rat serum treatment, the cell apoptosis rate decreased from (8.26+/-1.75)% to (2.75+/-0.90)%, while the concentration of [Ca2+]i increased from (96.98+/-3.95) to (118.79+/-3.22) nmol/L in serum from crush injury rats, respectively. The concentration of ET-1 and ANP increased significantly in crush injury rat serum.@*CONCLUSION@#Serum from crush injury rats could inhibit apoptosis of the vascular endothelial cells. These effects may be related to increased level of [Ca2+]i mediated by ET-1 and ANP.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cells, Cultured
/
Calcium
/
Atrial Natriuretic Factor
/
Rats, Sprague-Dawley
/
Apoptosis
/
Endothelin-1
/
Culture Media
/
Endothelial Cells
/
Disease Models, Animal
/
Extremities
Limits:
Animals
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2007
Type:
Article
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