Application of immunofluorescence and sandwich ELISA with double-antibodies in detection of human rabies / 法医学杂志
Journal of Forensic Medicine
;
(6): 411-413, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-983328
ABSTRACT
OBJECTIVE@#To explore the application of immunofluorescence and sandwich ELISA with double-antibodies in detection of human rabies.@*METHODS@#The cerebrum, cerebellum, brainstem, and hippocampus of four patients died of rabies identified by clinical diagnosis were collected and kept in freezer at -70 degrees C or in formaldehyde solution separately. The rat brain tissue infected by CVS strain of rabies virus was used as a positive control and the brain tissue of a patient died of acute pancreatitis was used as a negative control.@*RESULTS@#Rabies virus was detected in the tissues kept in freezer at -70 degrees C and the positive control but was not detected in the tissues kept in formaldehyde solution and the negative control.@*CONCLUSION@#Immunofluorescence and Sandwich ELISA with double-antibodies could be used in detection of human rabies. The samples should be kept in deep frozen temperature condition instead of in formaldehyde solution.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Rabies
/
Rabies virus
/
Reagent Kits, Diagnostic
/
Tissue Preservation
/
Brain
/
Enzyme-Linked Immunosorbent Assay
/
Fluorescent Antibody Technique
/
Sensitivity and Specificity
/
Hippocampus
/
Antibodies, Viral
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2007
Type:
Article
Similar
MEDLINE
...
LILACS
LIS