Age-dependent mitochondrial DNA 4977bp depletion in human skeletal muscle / 法医学杂志
Journal of Forensic Medicine
;
(6): 438-440, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-983335
ABSTRACT
OBJECTIVE@#To explore depletion of human mitochondrial DNA 4977-bp and its relation with aging.@*METHODS@#Total DNA (nuclear and mtDNA) was extracted from 100mg muscle tissue. UV light illumination of ethidium bromide-stained PCR products was used to study the depletion of mtDNA (wild-type or mutant).@*RESULTS@#The proportions of mtDNA depletion in human skeletal muscle could be determined. The frequency of mtDNA 4977-bp depletion in different age groups (0-9, 10-19, 20-29, 30-39, 40-49, 50-59, 60-69, 70-79, 80-89, 90-99) was 0%, 0%, 0.003%, 0.011%, 0.015%, 0.033%, 0.038%, 0.062%, 0.069%, and 0.091%, respectively.@*CONCLUSION@#Our findings suggest that the frequency of the mtDNA4977 depletion in human skeletal muscle increases with age. It might be useful for human age estimation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aging
/
DNA, Mitochondrial
/
Polymerase Chain Reaction
/
Age Factors
/
Sequence Deletion
/
DNA Primers
/
Muscle, Skeletal
/
Mitochondria, Muscle
Limits:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2007
Type:
Article
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