Time-dependent expression of caspase-3 during contused skeletal muscle wound healing in rats / 法医学杂志
Journal of Forensic Medicine
;
(6): 5-11, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-983343
ABSTRACT
OBJECTIVE@#To investigate the expression of caspase-3 during contused skeletal muscle wound healing in rats and to explore the applicability of caspase-3 in wound age estimation.@*METHODS@#Male Sprague-Dawley rats undergone anesthesia with 2% pentobarbital sodium (45 mg/kg) were struck by a single strike at the skeletal muscle of the right leg, then were sacrificed by cervical dislocation at different post-traumatic intervals. The changes of caspase-3 activity were assessed by imunohistochemistry, Western blot and caspase-3 colorimetric assay. The skeletal muscle of non-contusion rat's were used as controls.@*RESULTS@#No caspase-3 was detected in skeletal muscle in the control group. The expression level of caspase-3 in the peripheral area of contused rat skeletal muscle increased gradually and maximized on the fifth day after injury, and then decreased gradually. Caspase-3 was also detected in the regenerated skeletal muscle within 7-14 days after injury.@*CONCLUSION@#The expression of capase-3 in the peripheral area of contused rat skeletal muscle indicated that it may play a pivotal role in apoptosis of the wounded skeletal muscle and may be used as a marker for wound age estimation. The expression of capase-3 in regenerating skeletal muscle suggests that caspase-3 activity may be used for skeletal muscle differentiation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Time Factors
/
Wound Healing
/
Random Allocation
/
Rats, Sprague-Dawley
/
Muscle, Skeletal
/
Contusions
/
Forensic Pathology
/
Caspase 3
Limits:
Animals
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2008
Type:
Article
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