DNA typing of minimal oral epithelial cell samples / 法医学杂志
Journal of Forensic Medicine
;
(6): 126-128, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-983367
ABSTRACT
OBJECTIVE@#To seek simple and cost-effective extraction technique to improve multiplex STR amplification from minimal oral epithelial cell samples.@*METHODS@#One hundred DNA samples of oral epithelial cells extracted by mini system Chelex-100 method were quantitated by ABI 7500 Real Time System and were then typed with Identifiler system in ABI 3130 Genetic Analyzer.@*RESULTS@#The DNA contents of different categories of samples were as followings suck pipes (0.72-116.78 ng), cup edges (2.15-142.5 ng), mouths of drink bottle (1-34.65 ng), chopsticks (3.35-26.6 ng), fruit cores (0.294-21.4 ng), and poultry bones (0.88-5.88 ng). The mean successful typing rate for gender and more than 9 STR loci was about 59.38%. Except the addition or no addition of proteinase K to the samples, all other factors-C users' variation, sample extraction methods, and qualities and properties of the samples had considerable effects on the contents of extracted DNA.@*CONCLUSION@#Successful STR typing can be achieved in about 60% minimal oral epithelial cell DNA samples extracted by mini Chelex system.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA
/
Polymerase Chain Reaction
/
Microsatellite Repeats
/
Epithelial Cells
/
Forensic Medicine
/
Mouth Mucosa
Limits:
Humans
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2008
Type:
Article
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