Analysis of appropriate amount of template DNA for sinofiler kit by real time quantitative PCR technique / 法医学杂志

ABSTRACT

OBJECTIVE@#To explore the appropriate amount of template DNA for Sinofiler Kit.@*METHODS@#The DNA samples with ideally genotyped results by Sinofiler Kit were detected by real-time quantitative PCR assay.@*RESULTS@#It was shown that 1.29-1.51 ng of template DNA in 12.5 microL reaction volume was optimal for STR genotyping with Sinofiler Kit.@*CONCLUSION@#Real time quantitative PCR is an accurate and necessary technique for detection of appropriate amount of template DNA for different kits.