The time-dependent changes of phospho-JNK expression during the skin incised wound healing in mice / 法医学杂志
Journal of Forensic Medicine
;
(6): 241-244, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-983385
ABSTRACT
OBJECTIVE@#To investigate the changes of phospho-JNK (p-JNK) during the incised wound healing of the skin in mice and to explore the rule of the time-dependent change of p-JNK in wound age determination.@*METHODS@#The changes of p-JNK expression in incised skin wound were detected by immunohistochemistry and Western blot.@*RESULTS@#There was a minimal baseline staining of p-JNK in control mouse skin. Changes of p-JNK expression were mainly detectable in neutrophils in the wound specimens from 3 hours to 12 hours after injury. Afterwards, the p-JNK positive cells were mostly mononuclear cells and fibroblasts between post-injury day 1 and day 5, whereas the p-JNK positive cells were mostly fibroblasts between post-injury day 7 and day 14. Morphometrically, the ratio of the p-JNK positive cells to the total increased gradually in the wound specimens from 3 hours to day 1, and maximized at day 1 with a slight decrease from post-injury day 3 to day 5. The ratio showed a second peak in the specimens of day 7, and then decreased gradually from post-injury day 10 to day 14. The changes of p-JNK expression were observed throughout the wound healing stages by Western blot as well, with a peak expression occurring between 12 hour and day 3 after injury.@*CONCLUSION@#p-JNK may play a pivotal role in inducing apoptosis of neutrophils, mononuclear cells, and fibroblasts during skin wound healing and meanwhile, p-JNK may be a potentially useful marker for wound age determination.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphorylation
/
Skin
/
Time Factors
/
Wound Healing
/
Wounds, Penetrating
/
Biomarkers
/
Random Allocation
/
JNK Mitogen-Activated Protein Kinases
/
Forensic Medicine
Limits:
Animals
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2008
Type:
Article
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