Multiple amplification of 16S rRNA gene and Cytb gene in mitochondrial DNA for species identification / 法医学杂志
Journal of Forensic Medicine
;
(6): 259-261, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-983390
ABSTRACT
OBJECTIVE@#To establish a fluorescent multiple amplification system of 16S rRNA and Cytb genes located in mitochondrial DNA for species identification.@*METHODS@#A pair of primers of 16S rRNA gene and Cytb gene of the mitochondrial DNA was designed with the software Primer 5.0 to construct a multiple amplification system. The amplified products from human and five species of animals, including cattle, pig, dog, chicken and grass carp were analyzed by 310 Genetic Analyzer.@*RESULTS@#The amplified products of these samples showed two peaks. The common one was 358bp and the specific one different in unique species was between 231bp and 256bp.@*CONCLUSION@#The multiplex amplification system can exactly distinguish the species of human from five common animals.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Species Specificity
/
Swine
/
DNA, Mitochondrial
/
Carps
/
Molecular Sequence Data
/
RNA, Ribosomal, 16S
/
Base Sequence
/
Chickens
/
Gene Amplification
/
Cytochromes b
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2008
Type:
Article
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