DNA polymorphism detection of Papaver somniferum L using fluorescent amplified fragment length polymorphism / 法医学杂志
Journal of Forensic Medicine
; (6): 262-267, 2008.
Article
in Zh
| WPRIM
| ID: wpr-983391
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To detect DNA polymorphism of Papaver somniferum L using fluorescent Amplified Fragment Length Polymorphism.@*METHODS@#Genomic DNA was isolated using the AxyPrep DNA Kit, double-digested by two restrictional endonucleases (Eco RI and Mse I) and ligated to oligonucleotide adapters. After Pre-amplification and selective amplification, the DNA fragments were separated by capillary electrophoresis using the CEQ8000 DNA Fragment Analyzer.@*RESULTS@#More than 20 fragments of highly polymorphic products were obtained by 8 pairs of primer from 64 selective amplifying primer pairs.@*CONCLUSION@#The fluorescent AFLP technique can be used to detect the DNA polymorphism of Papaver somniferum.
Full text:
1
Index:
WPRIM
Main subject:
Papaver
/
Polymorphism, Genetic
/
DNA, Plant
/
Forensic Genetics
/
Amplified Fragment Length Polymorphism Analysis
/
Fluorescent Dyes
Language:
Zh
Journal:
Journal of Forensic Medicine
Year:
2008
Type:
Article