The application of three methods combined for extracting DNA from human epithelium / 法医学杂志

ABSTRACT

OBJECTIVE@#To improve DNA extraction from human epithelium.@*METHODS@#Chelex-100, Chelex-100 organic combined and Chelex-100 magnetism pearl combined methods were adopted according to the situation of each case.@*RESULTS@#With the stepwise extracting protocol, the trace amount of DNA was analyzed most efficiently and the success rate of DNA extracting was better improved.@*CONCLUSION@#The efficiency of DNA extraction from human epithelium could be greatly improved by modifying the extracting protocol such as purification and concentration based on previous results.