Simultaneous determination of clozapine, olanzapine and mirtazapine in human plasma by LC-MS/MS / 法医学杂志
Journal of Forensic Medicine
;
(6): 123-126, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-983458
ABSTRACT
OBJECTIVE@#To develop a method for determination of clozapine, olanzapine and mirtazapine in human plasma by liquid chromatography-tandem mass spectrometry(LC-MS/MS).@*METHODS@#Clozapine, olanzapine and mirtazapine were extracted from plasma samples by using diethyl ether and separated by Agilent Zorbax SB-C18 column(2.1 mm x 150 mm, 5 microm). Electrospray ionization source was applied, positive ion mode was used to detect and multiple reaction monitoring mode was used to quantify clozapine, olanzapine and mirtazapine. Carbamazepine was the internal standard.@*RESULTS@#The detection limits of clozapine, olanzapine and mirtazapine were within 0.41-0.92 ng/mL. The calibration curve in the concentration range of 10.0-2000.0 ng/mL showed a good linear distribution (r > or = 0.992 4). The average extraction recoveries were within 65.7%-94.2%. Intra-day RSD and inter-day RSD were less than 6% (n = 5).@*CONCLUSION@#This method seems to be quite specific, sensitive and accurate, and can be used to detect clozapine, olanzapine and mirtazapine in forensic and clinical analytic toxicology.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Benzodiazepines
/
Chromatography, Liquid
/
Clozapine
/
Tandem Mass Spectrometry
/
Forensic Toxicology
/
Olanzapine
/
Mirtazapine
/
Mianserin
Limits:
Humans
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2009
Type:
Article
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