Changes of ICAM-1 mRNA expression in rats' skeletal muscle after contusion / 法医学杂志
Journal of Forensic Medicine
;
(6): 161-163, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-983463
ABSTRACT
OBJECTIVE@#To investigate the expression of intercellular adhesion molecular-1 (ICAM-1) mRNA in contused skeletal muscle of rats using fluorescence in situ hybridization (FISH) and its relationship with the contusion interval.@*METHODS@#To make the contusion models with rats skeletal muscle. The samples were taken to extract mRNA at 0.5 h, 1 h, 6 h, 12 h, 18 h, 24 h, 30 h and from the control group after contusion respectively. FISH was performed on frozen section samples and the sections were observed using LSCM.@*RESULTS@#The expression of ICAM-1 mRNA peaked at 6 h in skeletal muscle after contusion. Its level fell to 3.46 times the level of control group at 18 h and then increased again.@*CONCLUSION@#The time-order expression of ICAM-1 mRNA in 30 hours after contusion is potentially useful for estimation of early wound age.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Time Factors
/
Wound Healing
/
RNA, Messenger
/
Random Allocation
/
Rats, Sprague-Dawley
/
In Situ Hybridization, Fluorescence
/
Muscle, Skeletal
/
Intercellular Adhesion Molecule-1
/
Contusions
/
Forensic Pathology
Limits:
Animals
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2009
Type:
Article
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