Expression of FAP and alpha-SMA during the incised wound healing in mice skin / 法医学杂志
Journal of Forensic Medicine
;
(6): 405-408, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-983512
ABSTRACT
UNLABELLED@#OBJECTIVE To investigate the time-dependent expression of fibroblast activation protein (FAP) and alpha-smooth muscle actin(alpha-SMA) during the incised wound healing of the skin in mice.@*METHODS@#The expression of FAP and alpha-SMA in incised wound of mice skin was detected by immunohistochemistry and Western blot.@*RESULTS@#By immunohistochemistry, the expression of FAP and alpha-SMA in the normal skin and the skin 1 h after injury maintained at a very low level, but the positive cells expressing FAP and alpha-SMA started to elevate 6 h after injury and reached its peak on 5 d for FAP and on 3 d for alpha-SMA, then gradually decreased to the normal level on 14 d. The expression of FAP and alpha-SMA was observed throughout the wound healing stages 1 d after injuries by Western blot as well with a peak expression occurring on 5 d for FAP and on 3 d for alpha-SMA after injury.@*CONCLUSION@#FAP may be a potentially useful marker for wound age determination and alpha-SMA may be used as an effective indicator for the mid- and late stage incised wound of mice skin. The combination use of FAP and alpha-SMA may be potentially effective indicators for wound age determination.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Endopeptidases
/
Skin
/
Time Factors
/
Wound Healing
/
Wounds and Injuries
/
Immunohistochemistry
/
Serine Endopeptidases
/
Random Allocation
/
Blotting, Western
/
Actins
Limits:
Animals
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2009
Type:
Article
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