Development and application of X-chromosomal STR multiplex amplification system / 法医学杂志

ABSTRACT

OBJECTIVE@#To explore the application of X-chromosomal STR(X-STR) for forensic identification and paternity testing.@*METHODS@#Six X-STR loci DXS6801, DXS9902, DXS6809, DXS6803, DXS6804 and DXS6799 were amplified in a single PCR reaction. PCR products were analyzed using capillary electrophoresis and 3100 Genetic Analyzer and GeneMapper ID v3.1 Analysis Software.@*RESULTS@#The alleles of all six X-STR loci were successfully obtained with unambiguous genotyping, high sensitivity and reproducibility.@*CONCLUSION@#The multiplex PCR of six X-STR loci is useful in forensic identification, particularly for sisters cases.