X-sNP genotyping using the TaqMan probe technology / 法医学杂志
Journal of Forensic Medicine
;
(6): 22-25, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-983532
ABSTRACT
OBJECTIVE@#To develop a rapid, accurate and economical real time fluorescence PCR method with TaqMan probe technology to detect the X chromosome single nucleotide polymorphism (X-SNP).@*METHODS@#TaqMan probes and polymerase chain reaction primers were respectively designed according to the 13 X-SNP. Then, the X-SNP were genotyped after the amplification by real time fluorescence PCR.@*RESULTS@#All the loci follow the Hardy-Weinberg equilibrium. The polymorphic information content for 13 distinct loci varied between 0.3497 and 0.3750 while the heterozygosity ranged from 0.4537 to 0.5021. A real time fluorescent PCR method based on TaqMan probe was successfully developed and the results were accordant with those analyzed by DNA sequencing of the 13 X-SNP.@*CONCLUSION@#The allele specific real time fluorescence PCR based on TaqMan probe is a sensitive, simple technology and suitable for rapid analysis of XSNP. All the loci show highly polymorphic and may be potential in forensic genetics.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA
/
DNA Probes
/
China
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
/
Taq Polymerase
/
Polymorphism, Single Nucleotide
/
Chromosomes, Human, X
/
Asian People
/
Alleles
Limits:
Female
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2010
Type:
Article
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