Application of multiple polymorphism genetic markers in determination of half sibling sharing a same mother / 法医学杂志
Journal of Forensic Medicine
;
(6): 279-300, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-983580
ABSTRACT
OBJECTIVE@#Determination strategies for half sibling sharing a same mother were investigated through the detection of autosomal and X-chromosomal STR (X-STR) loci and polymorphisms on hypervariable (HV) region of mitochondrial DNA (mtDNA).@*METHODS@#Genomic DNA were extracted from blood stain samples of the 3 full siblings and one dubious half sibling sharing the same mother with them. Fifteen autosomal STR loci were genotyped by Sinofiler kit, and 19 X-STR loci were genotyped by Mentype Argus X-8 kit and 16 plex in-house system. Polymorphisms of mtDNA HV-I and HV-II were also detected with sequencing technology.@*RESULTS@#Full sibling relationship between the dubious half sibling and each of the 3 full siblings were excluded based on the results of autosomal STR genotyping and calculation of full sibling index (FSI) and half sibling index (HIS). Results of sequencing for mtDNA HV-I and HV-II showed that all of the 4 samples came from a same maternal line. X-STR genotyping results determined that the dubious half sibling shared a same mother with the 3 full siblings.@*CONCLUSION@#It is reliable to combine three different genotyping technologies including autosomal STR, X-STR and sequencing of mtDNA HV-I and HV-II for determination of half sibling sharing a same mother.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymorphism, Genetic
/
DNA, Mitochondrial
/
Genetic Markers
/
Sequence Analysis, DNA
/
Tandem Repeat Sequences
/
Chromosomes, Human, X
/
Siblings
/
Forensic Genetics
/
Genotype
Limits:
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2010
Type:
Article
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