Determination of bloodstain formation time by RNA analysis / 法医学杂志
Journal of Forensic Medicine
;
(6): 340-342, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-983591
ABSTRACT
OBJECTIVE@#To investigate the expression of 18S rRNA and beta-actin mRNA in bloodstain between 8 and 15 days after death and extrapolate the time of bloodstain formation.@*METHODS@#RNA in dried bloodstain at different times was extracted, then quantified for 18S rRNA and beta-actin mRNA by real-time RT-PCR. The bloodstain formation time was deduced based on the changes of the ratio of 18S rRNA to beta-actin mRNA at different time points.@*RESULTS@#The ratio of 18S rRNA to beta-actin mRNA increased gradually with time, indicating that rRNA and mRNA degraded in different rate with time. CONCLUSION; The ratio of 18S rRNA to beta-actin mRNA could be used for estimating the time of bloodstain formation in some period.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Postmortem Changes
/
Time Factors
/
Blood Stains
/
RNA, Messenger
/
RNA, Ribosomal, 18S
/
Actins
/
DNA, Complementary
/
Real-Time Polymerase Chain Reaction
/
Forensic Medicine
Limits:
Humans
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2010
Type:
Article
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