The relationship between GAPDH mRNA degradation in the mouse liver and postmortem interval / 法医学杂志
Journal of Forensic Medicine
;
(6): 425-427, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-983605
ABSTRACT
OBJECTIVE@#To explore the relationship between degradation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in the mouse liver and postmortem interval (PMI).@*METHODS@#Sixty NIH mice were sacrificed by cervical dislocation and suffocation, and then placed into 10 degrees C and 25 degrees C temperature-controlling systems. The changes of GAPDH mRNA in the liver were detected by two-step fluorimetric reverse transcriptase polymerase chain reaction (RT-PCR) technique and nucleic acids protein cryoscope from 0 to 48 h postmortem.@*RESULTS@#In the mouse liver, the amplification products of GAPDH mRNA could be examined within 48 h postmortem in 10 degrees C temperature-controlling system and within 36 h postmortem in 25 degrees C temperature-controlling system. The amplification products showed a decreasing tendency.@*CONCLUSION@#Degradation of GAPDH mRNA in the mouse liver is negative correlation with PMI. GAPDH mRNA could be a new marker for estimation of PMI.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Postmortem Changes
/
Temperature
/
Time Factors
/
RNA, Messenger
/
Regression Analysis
/
Reverse Transcriptase Polymerase Chain Reaction
/
RNA Stability
/
Forensic Pathology
/
Glyceraldehyde-3-Phosphate Dehydrogenases
/
Liver
Limits:
Animals
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2010
Type:
Article
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