Time-dependent recruitment and differentiation of fibrocytes in mouse skin wound healing / 法医学杂志

ABSTRACT

OBJECTIVE@#To investigate the time-dependent recruitment and differentiation of fibrocytes in skin wound healing.@*METHODS@#Fibrocytes (expressing CD45 and procollagen I ) and myofibroblasts (expressing CD45 and alpha-SMA) were co-localized by immunofluorescent staining. The number of fibrocytes and myofibroblasts was counted at different post-wounding interval.@*RESULTS@#At 3 d after injury, fibrocytes started to recruit at the margin of the wounds. At 5 d after injury, myofibroblasts started to appear in new formed granulation tissue. The number of fibrocytes and myofibroblasts peaked at 7 d post-wounding.@*CONCLUSION@#During skin wound healing, myofibroblasts in granulation tissue originated at least partly from fibrocytic differentiation. The time-dependent recruitment and differentiation of fibrocytes may provide new information for wound age determination.